- DE
Archive
- Recommendations for accurate in-gel DNA quantification
- Loading of protein ladders/markers
- Removal of genomic DNA from RNA preparations
- SDS-PAGE for small proteins
- Purification of genomic DNA from mouse tails with Proteinase K
- siRNA-DNA Cotransfection
- General recommendations for RNA electrophoresis
- General recommendations to avoid RNase contamination
- General considerations for transfection
- Protocol for PCR Product Clean-up
- Guidelines for Preventing Contamination of PCR
- Gel extraction of DNA fragments running close together on your agarosegel
- Troubleshooting Guide for PCR
- Determination of RNA fragment lenghts in Northern Blots without labelled markers
- Maxima™ SYBR Green qPCR Master Mix (2X)
- Fast simultaneous plasmid vector linearization and dephosphorylation
- Migration patterns of Spectra™ Multicolor Broad Range Protein Ladder
- Troubleshooting for Transfection
Tip
Sep 2010 Recommendations for accurate in-gel DNA quantification
- dNTPs, oligonucleotides, genomic DNA, RNA, NTPs or buffer components can interfere with spectrophotometrical measurements and may lead to inaccurate quantification of sample DNA. In these cases, it is best to rely on gel quantification data.
- For the most accurate quantification, use video-densitometry analysis.
- Use the same DNA loading dye solution (supplied with the DNA ladder/marker) for both the sample DNA and the ladder/marker DNA.
- Compare the sample band intensity with the ladder band of the closest size.
- If possible, adjust the concentration of the sample to approximately equalize it with the amount of DNA in the nearest band
